› 1jnb

X-ray diffraction
3.2Å resolution

CONNECTOR PROTEIN FROM BACTERIOPHAGE PHI29

Released:
DOI: 10.2210/pdb1jnb/pdb
PDB entry 1jnb coloured by chain, front view.
PDB entry 1jnb coloured by chain, side view.
PDB entry 1jnb coloured by chain, top view.
Source organism: Bacillus virus phi29
Primary publication:
Structure determination of the head-tail connector of bacteriophage phi29.
Simpson AA, Leiman PG, Tao Y, He Y, Badasso MO, Jardine PJ, Anderson DL, Rossmann MG
Acta Crystallogr D Biol Crystallogr 57 1260-9 (2001)
PMID: 11526317

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Portal protein Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 309 amino acids
Theoretical weight: 35.92 KDa
Source organism: Bacillus virus phi29
Expression system: Escherichia coli
UniProt:
  • Canonical: P04332 (Residues: 1-309; Coverage: 100%)
Gene name: 10
Sequence domains: Phage Connector (GP10)
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2
Unit cell:
a: 177.16Å b: 169.24Å c: 185.44Å
α: 90° β: 114.1° γ: 90°
R-values:
R R work R free
0.36 0.284 0.301
Expression system: Escherichia coli

Quick links

Citations

8 review citations

DNA packaging and delivery machines in tailed bacteriophages.
Johnson et al. (2007)
7 more

4 mentions without citation

Breaking Symmetry in Viral Icosahedral Capsids as Seen through the Lenses of X-ray Crystallography and Cryo-Electron Microscopy.
Parent et al. (2018)
3 more