› 1jo8

X-ray diffraction
1.3Å resolution

Structural analysis of the yeast actin binding protein Abp1 SH3 domain

Released:
DOI: 10.2210/pdb1jo8/pdb
PDB entry 1jo8 coloured by chain, front view.
PDB entry 1jo8 coloured by chain, side view.
PDB entry 1jo8 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Unusual binding properties of the SH3 domain of the yeast actin-binding protein Abp1: structural and functional analysis.
Fazi B, Cope MJ, Douangamath A, Ferracuti S, Schirwitz K, Zucconi A, Drubin DG, Wilmanns M, Cesareni G, Castagnoli L
J Biol Chem 277 5290-8 (2002)
PMID: 11668184

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147521 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin-binding protein Chain: A
Molecule details ›
Chain: A
Length: 58 amino acids
Theoretical weight: 6.66 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P15891 (Residues: 535-592; Coverage: 10%)
Gene names: ABP1, YCR088W, YCR88W
Sequence domains: SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 24.08Å b: 38.155Å c: 59.09Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.144 0.178
Expression system: Escherichia coli

Quick links

Citations

10 review citations

The yeast actin cytoskeleton: from cellular function to biochemical mechanism.
Moseley et al. (2006)
9 more

21 mentions without citation

What lessons can be learned from studying the folding of homologous proteins?
Nickson et al. (2010)
20 more