› 1jps

X-ray diffraction
1.85Å resolution

Crystal structure of tissue factor in complex with humanized Fab D3h44

Released:
DOI: 10.2210/pdb1jps/pdb
PDB entry 1jps coloured by chain, front view.
PDB entry 1jps coloured by chain, side view.
PDB entry 1jps coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The 1.85 A resolution crystal structures of tissue factor in complex with humanized Fab D3h44 and of free humanized Fab D3h44: revisiting the solvation of antigen combining sites.
Faelber K, Kirchhofer D, Presta L, Kelley RF, Muller YA
J Mol Biol 313 83-97 (2001)
PMID: 11601848

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-233286 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
immunoglobulin Fab D3H44, light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
immunoglobulin Fab D3H44, heavy chain Chain: H
Molecule details ›
Chain: H
Length: 225 amino acids
Theoretical weight: 24.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Tissue factor Chain: T
Molecule details ›
Chain: T
Length: 219 amino acids
Theoretical weight: 24.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13726 (Residues: 33-251; Coverage: 83%)
Gene name: F3
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 84.25Å b: 93.28Å c: 110.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.224
Expression system: Escherichia coli

Quick links

Citations

17 citation in other articles

Localization and characterization of flavivirus envelope glycoprotein cross-reactive epitopes.
Crill et al. (2004)
16 more

33 mentions without citation

Prediction of residues in discontinuous B-cell epitopes using protein 3D structures.
Haste Andersen et al. (2006)
32 more