› 1jt0

X-ray diffraction
2.9Å resolution

Crystal structure of a cooperative QacR-DNA complex

Released:
DOI: 10.2210/pdb1jt0/pdb
PDB entry 1jt0 coloured by chain, front view.
PDB entry 1jt0 coloured by chain, side view.
PDB entry 1jt0 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structural basis for cooperative DNA binding by two dimers of the multidrug-binding protein QacR.
Schumacher MA, Miller MC, Grkovic S, Brown MH, Skurray RA, Brennan RG
EMBO J 21 1210-8 (2002)
PMID: 11867549

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114027 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
HTH-type transcriptional regulator QacR Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 194 amino acids
Theoretical weight: 22.98 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A0N4 (Residues: 1-188; Coverage: 100%)
Gene names: SAP100B_005, SAP104C_022, qacR
Sequence domains:
Structure domains:
QACA operator Chains: E, F
Molecule details ›
Chains: E, F
Length: 28 nucleotides
Theoretical weight: 8.6 KDa
Source organism: Staphylococcus aureus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P65
Unit cell:
a: 174.7Å b: 174.7Å c: 151.95Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.222 0.258
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

19 review citations

The TetR family of regulators.
Cuthbertson et al. (2013)
18 more

41 mentions without citation

The TetR family of transcriptional repressors.
Ramos et al. (2005)
40 more