› 1jwl

X-ray diffraction
4Å resolution

Structure of the Dimeric lac Repressor/Operator O1/ONPF Complex

Released:
DOI: 10.2210/pdb1jwl/pdb
PDB entry 1jwl coloured by chain, front view.
PDB entry 1jwl coloured by chain, side view.
PDB entry 1jwl coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystallographic analysis of Lac repressor bound to natural operator O1.
Bell CE, Lewis M
J Mol Biol 312 921-6 (2001)
PMID: 11580238

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-114036 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Lactose operon repressor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 333 amino acids
Theoretical weight: 35.76 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P03023 (Residues: 1-333; Coverage: 93%)
Gene names: JW0336, b0345, lacI
Sequence domains:
Structure domains:
5'-D(*AP*GP*AP*AP*T*TP*GP*TP*GP*AP*GP*CP*GP*GP*AP*TP*AP*AP*CP*AP*AP*TP*T)-3' Chain: D
Molecule details ›
Chain: D
Length: 23 nucleotides
Theoretical weight: 7.15 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*TP*AP*AP*TP*TP*GP*TP*TP*AP*TP*CP*CP*GP*CP*TP*CP*AP*CP*AP*AP*TP*TP*C)-3' Chain: E
Molecule details ›
Chain: E
Length: 23 nucleotides
Theoretical weight: 6.97 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NPF 3 x NPF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: R32
Unit cell:
a: 255.771Å b: 255.771Å c: 206.795Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.249 0.249 0.261
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

3 review citations

The lac repressor.
Lewis M. (2005)
2 more

7 mentions without citation

Modeling the Lac repressor-operator assembly: the influence of DNA looping on Lac repressor conformation.
Swigon et al. (2006)
6 more