› 1jwx

X-ray diffraction
1.63Å resolution

Chalcone Synthase--F215S mutant

Released:
DOI: 10.2210/pdb1jwx/pdb
PDB entry 1jwx coloured by chain, front view.
PDB entry 1jwx coloured by chain, side view.
PDB entry 1jwx coloured by chain, top view.
Source organism: Medicago sativa
Primary publication:
Expanding the biosynthetic repertoire of plant type III polyketide synthases by altering starter molecule specificity.
Jez JM, Bowman ME, Noel JP
Proc Natl Acad Sci U S A 99 5319-24 (2002)
PMID: 11959984

Function and Biology Details

Reaction catalysed: 
3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151628 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chalcone synthase 2 Chain: A
Molecule details ›
Chain: A
Length: 389 amino acids
Theoretical weight: 42.73 KDa
Source organism: Medicago sativa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30074 (Residues: 1-389; Coverage: 100%)
Gene name: CHS2
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CSD 1 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P3221
Unit cell:
a: 97.805Å b: 97.805Å c: 65.726Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.19 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

The biosynthetic logic of polyketide diversity.
Hertweck C. (2009)
26 more

2 mentions without citation

Structural classification and properties of ketoacyl synthases.
Chen et al. (2011)
1 more