› 1jx2

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF THE NUCLEOTIDE-FREE DYNAMIN A GTPASE DOMAIN, DETERMINED AS MYOSIN FUSION

Released:
DOI: 10.2210/pdb1jx2/pdb
PDB entry 1jx2 coloured by chain, front view.
PDB entry 1jx2 coloured by chain, side view.
PDB entry 1jx2 coloured by chain, top view.
Source organism: Dictyostelium discoideum
Primary publication:
Crystal structure of a dynamin GTPase domain in both nucleotide-free and GDP-bound forms.
Niemann HH, Knetsch ML, Scherer A, Manstein DJ, Kull FJ
EMBO J 20 5813-21 (2001)
PMID: 11689422

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140378 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dynamin-A; Myosin-2 heavy chain Chain: A
Molecule details ›
Chain: A
Length: 1100 amino acids
Theoretical weight: 124.57 KDa
Source organism: Dictyostelium discoideum
Expression system: Dictyostelium discoideum
UniProt:
  • Canonical: P08799 (Residues: 3-765; Coverage: 36%)
  • Canonical: Q94464 (Residues: 2-316; Coverage: 37%)
Gene names: DDB_G0277849, DDB_G0286355, dymA, mhcA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand BGC 1 x BGC
Ligand MG 1 x MG
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21
Unit cell:
a: 54.45Å b: 62.04Å c: 181.2Å
α: 90° β: 94.79° γ: 90°
R-values:
R R work R free
0.197 0.197 0.255
Expression system: Dictyostelium discoideum

Quick links

Citations

28 review citations

The dynamin superfamily: universal membrane tubulation and fission molecules?
Praefcke et al. (2004)
27 more

6 mentions without citation

Conformational changes in Dnm1 support a contractile mechanism for mitochondrial fission.
Mears et al. (2011)
5 more