› 1jye

X-ray diffraction
1.7Å resolution

Structure of a Dimeric Lac Repressor with C-terminal Deletion and K84L Substitution

Released:
DOI: 10.2210/pdb1jye/pdb
PDB entry 1jye coloured by chain, front view.
PDB entry 1jye coloured by chain, side view.
PDB entry 1jye coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of a variant of lac repressor with increased thermostability and decreased affinity for operator.
Bell CE, Barry J, Matthews KS, Lewis M
J Mol Biol 313 99-109 (2001)
PMID: 11601849

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136465 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactose operon repressor Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 37.43 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03023 (Residues: 1-349; Coverage: 97%)
Gene names: JW0336, b0345, lacI
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: I4122
Unit cell:
a: 87.007Å b: 87.007Å c: 224.098Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.241
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The lac repressor.
Lewis M. (2005)
3 more

4 mentions without citation

Engineering an allosteric transcription factor to respond to new ligands.
Taylor et al. (2016)
3 more