› 1k3r

X-ray diffraction
2.3Å resolution

Crystal Structure of the Methyltransferase with a Knot from Methanobacterium thermoautotrophicum

Released:
DOI: 10.2210/pdb1k3r/pdb
PDB entry 1k3r coloured by chain, front view.
PDB entry 1k3r coloured by chain, side view.
PDB entry 1k3r coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus
Primary publication:
Deep trefoil knot implicated in RNA binding found in an archaebacterial protein.
Zarembinski TI, Kim Y, Peterson K, Christendat D, Dharamsi A, Arrowsmith CH, Edwards AM, Joachimiak A
Proteins 50 177-83 (2003)
PMID: 12486711

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127817 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Conserved protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 268 amino acids
Theoretical weight: 30.24 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O26109 (Residues: 1-268; Coverage: 100%)
Gene name: MTH_1
Sequence domains: Putative RNA methyltransferase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID, null
Spacegroup: C2
Unit cell:
a: 101.307Å b: 51.353Å c: 109.25Å
α: 90° β: 94.69° γ: 90°
R-values:
R R work R free
0.24 0.221 0.277
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Protein knots and fold complexity: some new twists.
Taylor WR. (2007)
3 more

10 mentions without citation

The protein composition of mitotic chromosomes determined using multiclassifier combinatorial proteomics.
Ohta et al. (2010)
9 more