› 1k5u

X-ray diffraction
2Å resolution

Human acidic fibroblast growth factor. 141 amino acid form with amino terminal His tag with His93 replaced by Gly (H93G).

Released:
DOI: 10.2210/pdb1k5u/pdb
PDB entry 1k5u coloured by chain, front view.
PDB entry 1k5u coloured by chain, side view.
PDB entry 1k5u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Alternative type I and I' turn conformations in the beta8/beta9 beta-hairpin of human acidic fibroblast growth factor.
Kim J, Blaber SI, Blaber M
Protein Sci 11 459-66 (2002)
PMID: 11847269

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fibroblast growth factor 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 146 amino acids
Theoretical weight: 16.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05230 (Residues: 16-154; Coverage: 90%)
Gene names: FGF1, FGFA
Sequence domains: Fibroblast growth factor
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 33.63Å b: 97.71Å c: 57.46Å
α: 90° β: 104.3° γ: 90°
R-values:
R R work R free
0.233 0.219 0.293
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Highly stable mutants of human fibroblast growth factor-1 exhibit prolonged biological action.
Zakrzewska et al. (2005)
4 more

1 mention without citation

Electrostatic Forces as Dominant Interactions Between Proteins and Polyanions: an ESI MS Study of Fibroblast Growth Factor Binding to Heparin Oligomers.
Minsky et al. (2017)