› 1k61

X-ray diffraction
2.1Å resolution

MATALPHA2 HOMEODOMAIN BOUND TO DNA

Released:
DOI: 10.2210/pdb1k61/pdb
PDB entry 1k61 coloured by chain, front view.
PDB entry 1k61 coloured by chain, side view.
PDB entry 1k61 coloured by chain, top view.
Source organisms:
Primary publication:
A Hoogsteen base pair embedded in undistorted B-DNA.
Aishima J, Gitti RK, Noah JE, Gan HH, Schlick T, Wolberger C
Nucleic Acids Res 30 5244-52 (2002)
PMID: 12466549

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114053 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Mating-type protein ALPHA2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 60 amino acids
Theoretical weight: 7.28 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P0CY08 (Residues: 132-191; Coverage: 29%)
Gene names: ALPHA-2, MAT2A, MATAL2, MATALPHA2, YCR039C, YCR39C
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
5'-D(*AP*CP*AP*TP*GP*TP*AP*AP*TP*TP*CP*AP*TP*TP*TP*AP*CP*AP*CP*GP*C)-3' Chain: E
Molecule details ›
Chain: E
Length: 21 nucleotides
Theoretical weight: 6.38 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
5'-D(*(5IU)P*GP*CP*GP*TP*GP*TP*AP*AP*AP*TP*GP*AP*AP*TP*TP*AP*CP*AP*TP*G)-3' Chain: F
Molecule details ›
Chain: F
Length: 21 nucleotides
Theoretical weight: 6.61 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 5IU 1 x 5IU

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 38.94Å b: 70.24Å c: 68.29Å
α: 90° β: 105.42° γ: 90°
R-values:
R R work R free
0.221 0.221 0.277
Expression system: Not provided

Quick links

Citations

9 review citations

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
8 more

20 mentions without citation

Homeodomain revisited: a lesson from disease-causing mutations.
Chi YI. (2005)
19 more