› 1k66

X-ray diffraction
1.75Å resolution

Crystal Structure of the Cyanobacterial Phytochrome Response Regulator, RcpB

Released:
DOI: 10.2210/pdb1k66/pdb
PDB entry 1k66 coloured by chain, front view.
PDB entry 1k66 coloured by chain, side view.
PDB entry 1k66 coloured by chain, top view.
Source organism: Tolypothrix sp. PCC 7601
Primary publication:
Crystal structures of two cyanobacterial response regulators in apo- and phosphorylated form reveal a novel dimerization motif of phytochrome-associated response regulators.
Benda C, Scheufler C, Tandeau de Marsac N, Gärtner W
Biophys J 87 476-87 (2004)
PMID: 15240481

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186046 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Response regulatory domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 149 amino acids
Theoretical weight: 16.8 KDa
Source organism: Tolypothrix sp. PCC 7601
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RTM8 (Residues: 2-149; Coverage: 99%)
Gene names: FDUTEX481_00770, rcpB
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P41212
Unit cell:
a: 72.18Å b: 72.18Å c: 142.782Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.25
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Molecular Mechanisms of Two-Component Signal Transduction.
Zschiedrich et al. (2016)
5 more

4 mentions without citation

Evolutionary trace annotation of protein function in the structural proteome.
Erdin et al. (2010)
3 more