› 1k6o

X-ray diffraction
3.19Å resolution

Crystal Structure of a Ternary SAP-1/SRF/c-fos SRE DNA Complex

Released:
DOI: 10.2210/pdb1k6o/pdb
PDB entry 1k6o coloured by chain, front view.
PDB entry 1k6o coloured by chain, side view.
PDB entry 1k6o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a ternary SAP-1/SRF/c-fos SRE DNA complex.
Mo Y, Ho W, Johnston K, Marmorstein R
J Mol Biol 314 495-506 (2001)
PMID: 11846562

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114054 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
ETS domain-containing protein Elk-4 Chain: A
Molecule details ›
Chain: A
Length: 93 amino acids
Theoretical weight: 11.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P28324 (Residues: 1-93; Coverage: 22%)
Gene names: ELK4, SAP1
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Serum response factor Chains: B, C
Molecule details ›
Chains: B, C
Length: 103 amino acids
Theoretical weight: 11.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11831 (Residues: 133-235; Coverage: 20%)
Gene name: SRF
Sequence domains: SRF-type transcription factor (DNA-binding and dimerisation domain)
Structure domains: Transcription factor, MADS-box
5'-D(*CP*AP*CP*AP*GP*GP*AP*TP*GP*TP*CP*CP*AP*TP*AP*TP*TP*AP*GP*GP*AP*CP*A)-3' Chain: D
Molecule details ›
Chain: D
Length: 23 nucleotides
Theoretical weight: 7.07 KDa
5'-D(*TP*GP*TP*CP*CP*TP*AP*AP*TP*AP*TP*GP*GP*AP*CP*AP*TP*CP*CP*TP*GP*TP*G)-3' Chain: E
Molecule details ›
Chain: E
Length: 23 nucleotides
Theoretical weight: 7.05 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P63
Unit cell:
a: 161.784Å b: 161.784Å c: 41.224Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.22 0.276
Expression system: Escherichia coli

Quick links

Citations

11 review citations

A census of human transcription factors: function, expression and evolution.
Vaquerizas et al. (2009)
10 more

9 mentions without citation

Recent advances in the structural molecular biology of Ets transcription factors: interactions, interfaces and inhibition.
Cooper et al. (2014)
8 more