1k8w

X-ray diffraction
1.85Å resolution

Crystal structure of the E. coli pseudouridine synthase TruB bound to a T stem-loop RNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-158002 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
tRNA pseudouridine synthase B Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 36.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P60340 (Residues: 7-314; Coverage: 98%)
Gene names: JW3135, b3166, truB, yhbA
Sequence domains:
Structure domains:
5'-R(*GP*GP*CP*AP*AP*CP*GP*GP*UP*(FHU)P*CP*GP*AP*UP*CP*CP*CP*GP*UP*UP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 22 nucleotides
Theoretical weight: 7.06 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2, RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 145.054Å b: 40.361Å c: 77.987Å
α: 90° β: 110.6° γ: 90°
R-values:
R R work R free
0.184 0.184 0.211
Expression systems:
  • Escherichia coli
  • Not provided