› 1kf2

X-ray diffraction
1.1Å resolution

Atomic Resolution Structure of RNase A at pH 5.2

Released:
DOI: 10.2210/pdb1kf2/pdb
PDB entry 1kf2 coloured by chain, front view.
PDB entry 1kf2 coloured by chain, side view.
PDB entry 1kf2 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Atomic resolution structures of ribonuclease A at six pH values.
Berisio R, Sica F, Lamzin VS, Wilson KS, Zagari A, Mazzarella L
Acta Crystallogr D Biol Crystallogr 58 441-50 (2002)
PMID: 11856829

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.71 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21
Unit cell:
a: 30.44Å b: 38.38Å c: 53.32Å
α: 90° β: 105.74° γ: 90°
R-values:
R R work R free
0.103 not available not available

Quick links

Citations

4 review citations

Intracellular pH sensors: design principles and functional significance.
Srivastava et al. (2007)
3 more

6 mentions without citation

Reviewing Challenges of Predicting Protein Melting Temperature Change Upon Mutation Through the Full Analysis of a Highly Detailed Dataset with High-Resolution Structures.
Louis et al. (2021)
5 more