› 1kg5

X-ray diffraction
1.35Å resolution

Crystal structure of the K142Q mutant of E.coli MutY (core fragment)

Released:
DOI: 10.2210/pdb1kg5/pdb
PDB entry 1kg5 coloured by chain, front view.
PDB entry 1kg5 coloured by chain, side view.
PDB entry 1kg5 coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Gilboa R, Kilshtein A, Zharkov DO, Kycia JH, Gerchman SE, Grollman AP, Shoham G

Function and Biology Details

Reaction catalysed: 
Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148180 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenine DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 225 amino acids
Theoretical weight: 25.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P17802 (Residues: 1-225; Coverage: 64%)
Gene names: JW2928, b2961, micA, mutY
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand SF4 1 x SF4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: C2
Unit cell:
a: 83.49Å b: 50.12Å c: 70.38Å
α: 90° β: 122.68° γ: 90°
R-values:
R R work R free
0.128 0.128 0.169
Expression system: Escherichia coli

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