› 1kjx

X-ray diffraction
2.6Å resolution

IMP Complex of E. Coli Adenylosuccinate Synthetase

Released:
DOI: 10.2210/pdb1kjx/pdb
PDB entry 1kjx coloured by chain, front view.
PDB entry 1kjx coloured by chain, side view.
PDB entry 1kjx coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
IMP Alone Organizes the Active Site of Adenylosuccinate Synthetase from Escherichia coli.
Hou Z, Wang W, Fromm HJ, Honzatko RB
J Biol Chem 277 5970-6 (2002)
PMID: 11741996

Function and Biology Details

Reaction catalysed: 
GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141584 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate synthetase Chain: A
Molecule details ›
Chain: A
Length: 432 amino acids
Theoretical weight: 47.4 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7D4 (Residues: 1-432; Coverage: 100%)
Gene names: JW4135, adeK, b4177, purA
Sequence domains: Adenylosuccinate synthetase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand IMP 1 x IMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P3221
Unit cell:
a: 80.6Å b: 80.6Å c: 158.65Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.196 0.283
Expression system: Escherichia coli

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Citations

7 citation in other articles

Chlamydomonas reinhardtii secretes compounds that mimic bacterial signals and interfere with quorum sensing regulation in bacteria.
Teplitski et al. (2004)
6 more