› 1knz

X-ray diffraction
2.45Å resolution

Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer

Released:
DOI: 10.2210/pdb1knz/pdb
PDB entry 1knz coloured by chain, front view.
PDB entry 1knz coloured by chain, side view.
PDB entry 1knz coloured by chain, top view.
Source organism: Simian rotavirus A/SA11
Primary publication:
Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer.
Deo RC, Groft CM, Rajashankar KR, Burley SK
Cell 108 71-81 (2002)
PMID: 11792322

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137116 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Non-structural protein 3 Chains: A, B, C, D, I, J, M, N
Molecule details ›
Chains: A, B, C, D, I, J, M, N
Length: 164 amino acids
Theoretical weight: 18.38 KDa
Source organism: Simian rotavirus A/SA11
Expression system: Escherichia coli
UniProt:
  • Canonical: P03536 (Residues: 4-164; Coverage: 51%)
Sequence domains: Rotavirus non-structural protein NSP3
Structure domains:
5'-R(*UP*GP*AP*CP*C)-3' Chains: W, X, Y, Z
Molecule details ›
Chains: W, X, Y, Z
Length: 5 nucleotides
Theoretical weight: 1.55 KDa
Source organism: Simian rotavirus A/SA11
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P1
Unit cell:
a: 40.275Å b: 85.359Å c: 96.051Å
α: 89.99° β: 90° γ: 90.05°
R-values:
R R work R free
0.228 0.228 0.28
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

15 review citations

Eukaryotic translation initiation factors and regulators.
Sonenberg et al. (2003)
14 more

12 mentions without citation

Rotavirus non-structural proteins: structure and function.
Hu et al. (2012)
11 more