› 1ko7

X-ray diffraction
1.95Å resolution

X-ray structure of the HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution

Released:
DOI: 10.2210/pdb1ko7/pdb
PDB entry 1ko7 coloured by chain, front view.
PDB entry 1ko7 coloured by chain, side view.
PDB entry 1ko7 coloured by chain, top view.
Source organism: Staphylococcus xylosus
Primary publication:
Structure of the full-length HPr kinase/phosphatase from Staphylococcus xylosus at 1.95 A resolution: Mimicking the product/substrate of the phospho transfer reactions.
Márquez JA, Hasenbein S, Koch B, Fieulaine S, Nessler S, Russell RB, Hengstenberg W, Scheffzek K
Proc Natl Acad Sci U S A 99 3458-63 (2002)
PMID: 11904409

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HPr kinase/phosphorylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 314 amino acids
Theoretical weight: 35.37 KDa
Source organism: Staphylococcus xylosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9S1H5 (Residues: 1-314; Coverage: 100%)
Gene name: hprK
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 6 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: R32
Unit cell:
a: 152.24Å b: 152.24Å c: 194.686Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.226 0.246
Expression system: Escherichia coli

Quick links

Citations

10 review citations

How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.
Deutscher et al. (2006)
9 more

8 mentions without citation

Structure-based phylogeny of the metallo-beta-lactamases.
Garau et al. (2005)
7 more