› 1ko9

X-ray diffraction
2.15Å resolution

Native Structure of the Human 8-oxoguanine DNA Glycosylase hOGG1

Released:
DOI: 10.2210/pdb1ko9/pdb
PDB entry 1ko9 coloured by chain, front view.
PDB entry 1ko9 coloured by chain, side view.
PDB entry 1ko9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Reciprocal "flipping" underlies substrate recognition and catalytic activation by the human 8-oxo-guanine DNA glycosylase.
Bjørås M, Seeberg E, Luna L, Pearl LH, Barrett TE
J Mol Biol 317 171-7 (2002)
PMID: 11902834

Function and Biology Details

Reaction catalysed: 
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127510 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-glycosylase/DNA lyase Chain: A
Molecule details ›
Chain: A
Length: 345 amino acids
Theoretical weight: 38.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15527 (Residues: 1-345; Coverage: 100%)
Gene names: MMH, MUTM, OGG1, OGH1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P65
Unit cell:
a: 105.513Å b: 105.513Å c: 47.597Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.206 0.252
Expression system: Escherichia coli

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Citations

15 review citations

Base excision repair and cancer.
Wallace et al. (2012)
14 more

17 mentions without citation

Recent advances in the structural mechanisms of DNA glycosylases.
Brooks et al. (2013)
16 more