1ksx

X-ray diffraction
3.2Å resolution

Crystal Structures of Two Intermediates in the Assembly of the Papillomavirus Replication Initiation Complex

Released:

Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-114093 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Replication protein E1 Chains: A, B, E, F, I, J, M, N
Molecule details ›
Chains: A, B, E, F, I, J, M, N
Length: 148 amino acids
Theoretical weight: 16.88 KDa
Source organism: Bovine papillomavirus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03116 (Residues: 159-303; Coverage: 24%)
Gene name: E1
Sequence domains: Papillomavirus E1, DNA-binding domain
Structure domains: Replication Protein E1; Chain: A,
E1 Recognition Sequence Chains: C, G, K, O
Molecule details ›
Chains: C, G, K, O
Length: 21 nucleotides
Theoretical weight: 6.44 KDa
Source organism: Bovine papillomavirus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P21
Unit cell:
a: 84.246Å b: 103.65Å c: 124.996Å
α: 90° β: 99.53° γ: 90°
R-values:
R R work R free
0.263 0.263 0.285
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided