› 1kut

X-ray diffraction
2.2Å resolution

Structural Genomics, Protein TM1243, (SAICAR synthetase)

Released:
DOI: 10.2210/pdb1kut/pdb
PDB entry 1kut coloured by chain, front view.
PDB entry 1kut coloured by chain, side view.
PDB entry 1kut coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Structure of SAICAR synthase from Thermotoga maritima at 2.2 angstroms reveals an unusual covalent dimer.
Zhang R, Skarina T, Evdokimova E, Edwards A, Savchenko A, Laskowski R, Cuff ME, Joachimiak A
Acta Crystallogr Sect F Struct Biol Cryst Commun 62 335-9 (2006)
PMID: 16582479

Function and Biology Details

Reaction catalysed: 
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194748 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylaminoimidazole-succinocarboxamide synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 26.51 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X0X0 (Residues: 1-230; Coverage: 100%)
Gene names: TM_1243, purC
Sequence domains: SAICAR synthetase
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 63.509Å b: 43.067Å c: 80.217Å
α: 90° β: 92.3° γ: 90°
R-values:
R R work R free
0.245 0.245 0.281
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The impact of extremophiles on structural genomics (and vice versa).
Jenney et al. (2008)
6 other articles

2 mentions without citation

Structure of SAICAR synthase from Thermotoga maritima at 2.2 angstroms reveals an unusual covalent dimer.
Zhang et al. (2006)
1 more