› 1ky9

X-ray diffraction
2.8Å resolution

Crystal Structure of DegP (HtrA)

Released:
DOI: 10.2210/pdb1ky9/pdb
PDB entry 1ky9 coloured by chain, front view.
PDB entry 1ky9 coloured by chain, side view.
PDB entry 1ky9 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of DegP (HtrA) reveals a new protease-chaperone machine.
Krojer T, Garrido-Franco M, Huber R, Ehrmann M, Clausen T
Nature 416 455-9 (2002)
PMID: 11919638

Function and Biology Details

Reaction catalysed: 
Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142926 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic serine endoprotease DegP Chains: A, B
Molecule details ›
Chains: A, B
Length: 448 amino acids
Theoretical weight: 47.51 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C0V0 (Residues: 27-474; Coverage: 100%)
Gene names: JW0157, b0161, degP, htrA, ptd
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P6322
Unit cell:
a: 121.374Å b: 121.374Å c: 233.667Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.218 0.275
Expression system: Escherichia coli

Quick links

Citations

52 review citations

IAP proteins: blocking the road to death's door.
Salvesen et al. (2002)
51 more

25 mentions without citation

Building Better Barrels - β-barrel Biogenesis and Insertion in Bacteria and Mitochondria.
Diederichs et al. (2021)
24 more