› 1kyr

X-ray diffraction
1.5Å resolution

Crystal Structure of a Cu-bound Green Fluorescent Protein Zn Biosensor

Released:
DOI: 10.2210/pdb1kyr/pdb
PDB entry 1kyr coloured by chain, front view.
PDB entry 1kyr coloured by chain, side view.
PDB entry 1kyr coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Structural chemistry of a green fluorescent protein Zn biosensor.
Barondeau DP, Kassmann CJ, Tainer JA, Getzoff ED
J Am Chem Soc 124 3522-4 (2002)
PMID: 11929238

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 26.71 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand CU 1 x CU
Ligand MG 1 x MG
1 modified residue:
Ligand CRG 1 x CRG

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 51.166Å b: 62.301Å c: 69.624Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.15 0.219
Expression system: Escherichia coli BL21(DE3)

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Citations

23 review citations

Creating new fluorescent probes for cell biology.
Zhang et al. (2002)
22 more