› 1l6f

X-ray diffraction
2Å resolution

Alanine racemase bound with N-(5'-phosphopyridoxyl)-L-alanine

Released:
DOI: 10.2210/pdb1l6f/pdb
PDB entry 1l6f coloured by chain, front view.
PDB entry 1l6f coloured by chain, side view.
PDB entry 1l6f coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Reaction mechanism of alanine racemase from Bacillus stearothermophilus: x-ray crystallographic studies of the enzyme bound with N-(5'-phosphopyridoxyl)alanine.
Watanabe A, Yoshimura T, Mikami B, Hayashi H, Kagamiyama H, Esaki N
J Biol Chem 277 19166-72 (2002)
PMID: 11886871

Function and Biology Details

Reaction catalysed: 
L-alanine = D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanine racemase Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 43.7 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10724 (Residues: 1-388; Coverage: 100%)
Gene names: alr, dal
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PP3 2 x PP3
1 modified residue:
Ligand KCX 2 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE
Spacegroup: P212121
Unit cell:
a: 99.96Å b: 90.866Å c: 85.417Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.172 0.225
Expression system: Escherichia coli

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Citations

11 review citations

Cytoplasmic steps of peptidoglycan biosynthesis.
Barreteau et al. (2008)
10 more

6 mentions without citation

Molecular mechanisms of vancomycin resistance.
Stogios et al. (2020)
5 more