› 1l8b

X-ray diffraction
1.8Å resolution

Cocrystal Structure of the Messenger RNA 5' Cap-binding Protein (eIF4E) bound to 7-methylGpppG

Released:
DOI: 10.2210/pdb1l8b/pdb
PDB entry 1l8b coloured by chain, front view.
PDB entry 1l8b coloured by chain, side view.
PDB entry 1l8b coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Biophysical studies of eIF4E cap-binding protein: recognition of mRNA 5' cap structure and synthetic fragments of eIF4G and 4E-BP1 proteins.
Niedzwiecka A, Marcotrigiano J, Stepinski J, Jankowska-Anyszka M, Wyslouch-Cieszynska A, Dadlez M, Gingras AC, Mak P, Darzynkiewicz E, Sonenberg N, Burley SK, Stolarski R
J Mol Biol 319 615-35 (2002)
PMID: 12054859

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158938 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic translation initiation factor 4E Chains: A, B
Molecule details ›
Chains: A, B
Length: 190 amino acids
Theoretical weight: 22.15 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63073 (Residues: 28-217; Coverage: 88%)
Gene name: Eif4e
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e

Ligands and Environments

1 bound ligand:
Ligand MGP 2 x MGP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 70.12Å b: 75.92Å c: 77.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

41 review citations

The molecular mechanics of eukaryotic translation.
Kapp et al. (2004)
40 more

19 mentions without citation

Regulation of mRNA translation by signaling pathways.
Roux et al. (2012)
18 more