› 1lev

X-ray diffraction
2.15Å resolution

PORCINE KIDNEY FRUCTOSE-1,6-BISPHOSPHATASE COMPLEXED WITH AN AMP-SITE INHIBITOR

Released:
DOI: 10.2210/pdb1lev/pdb
PDB entry 1lev coloured by chain, front view.
PDB entry 1lev coloured by chain, side view.
PDB entry 1lev coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
3-(2-carboxyethyl)-4,6-dichloro-1H-indole-2-carboxylic acid: an allosteric inhibitor of fructose-1,6-bisphosphatase at the AMP site.
Wright SW, Carlo AA, Danley DE, Hageman DL, Karam GA, Mansour MN, McClure LD, Pandit J, Schulte GK, Treadway JL, Wang IK, Bauer PH
Bioorg Med Chem Lett 13 2055-8 (2003)
PMID: 12781194

Function and Biology Details

Reaction catalysed: 
D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132778 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-1,6-bisphosphatase 1 Chains: A, F
Molecule details ›
Chains: A, F
Length: 337 amino acids
Theoretical weight: 36.71 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00636 (Residues: 2-338; Coverage: 100%)
Gene names: FBP, FBP1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand F6P 2 x F6P
Ligand MN 4 x MN
Ligand CLI 2 x CLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 118.87Å b: 73.446Å c: 78.025Å
α: 90° β: 106.3° γ: 90°
R-values:
R R work R free
0.196 0.193 0.255
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Emerging experimental therapeutics for bipolar disorder: insights from the molecular and cellular actions of current mood stabilizers.
Gould et al. (2004)
1 more

2 mentions without citation

Molden 2.0: quantum chemistry meets proteins.
Schaftenaar et al. (2017)
1 more