› 1ljl

X-ray diffraction
2.01Å resolution

Wild Type pI258 S. aureus arsenate reductase

Released:
DOI: 10.2210/pdb1ljl/pdb
PDB entry 1ljl coloured by chain, front view.
PDB entry 1ljl coloured by chain, side view.
PDB entry 1ljl coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
All intermediates of the arsenate reductase mechanism, including an intramolecular dynamic disulfide cascade.
Messens J, Martins JC, Van Belle K, Brosens E, Desmyter A, De Gieter M, Wieruszeski JM, Willem R, Wyns L, Zegers I
Proc Natl Acad Sci U S A 99 8506-11 (2002)
PMID: 12072565

Function and Biology Details

Reaction catalysed: 
Arsenate + thioredoxin = arsenite + thioredoxin disulfide + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140882 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arsenate reductase Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 14.83 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A006 (Residues: 1-131; Coverage: 100%)
Gene name: arsC
Sequence domains: Low molecular weight phosphotyrosine protein phosphatase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P212121
Unit cell:
a: 32.45Å b: 32.77Å c: 100.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.214 0.259
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Bacterial silver resistance: molecular biology and uses and misuses of silver compounds.
Silver S. (2003)
9 more

11 mentions without citation

Analysis and functional prediction of reactive cysteine residues.
Marino et al. (2012)
10 more