› 1lk9

X-ray diffraction
1.53Å resolution

The Three-dimensional Structure of Alliinase from Garlic

Released:
DOI: 10.2210/pdb1lk9/pdb
PDB entry 1lk9 coloured by chain, front view.
PDB entry 1lk9 coloured by chain, side view.
PDB entry 1lk9 coloured by chain, top view.
Source organism: Allium sativum
Primary publication:
The active principle of garlic at atomic resolution.
Kuettner EB, Hilgenfeld R, Weiss MS
J Biol Chem 277 46402-7 (2002)
PMID: 12235163

Function and Biology Details

Reaction catalysed: 
An S-alkyl-L-cysteine S-oxide = an alkyl sulfenate + 2-aminoacrylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Alliin lyase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 448 amino acids
Theoretical weight: 51.52 KDa
Source organism: Allium sativum
UniProt:
  • Canonical: Q01594 (Residues: 39-486; Coverage: 98%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
4 bound ligands:
Ligand SO4 11 x SO4
Ligand CL 2 x CL
Ligand EPE 2 x EPE
Ligand DHA 1 x DHA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 68.45Å b: 101.07Å c: 155.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.221

Quick links

Citations

7 review citations

Bioactive S-alk(en)yl cysteine sulfoxide metabolites in the genus Allium: the chemistry of potential therapeutic agents.
Rose et al. (2005)
6 more

3 mentions without citation

Rapid synthesis of auxin via a new tryptophan-dependent pathway is required for shade avoidance in plants.
Tao et al. (2008)
2 more