› 1lob

X-ray diffraction
2Å resolution

THREE-DIMENSIONAL STRUCTURES OF COMPLEXES OF LATHYRUS OCHRUS ISOLECTIN I WITH GLUCOSE AND MANNOSE: FINE SPECIFICITY OF THE MONOSACCHARIDE-BINDING SITE

Released:
DOI: 10.2210/pdb1lob/pdb
PDB entry 1lob coloured by chain, front view.
PDB entry 1lob coloured by chain, side view.
PDB entry 1lob coloured by chain, top view.
Source organism: Lathyrus ochrus
Primary publication:
Three-dimensional structures of complexes of Lathyrus ochrus isolectin I with glucose and mannose: fine specificity of the monosaccharide-binding site.
Bourne Y, Roussel A, Frey M, Rougé P, Fontecilla-Camps JC, Cambillau C
Proteins 8 365-76 (1990)
PMID: 2091026

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137409 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lectin beta-1 and beta-2 chains Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 181 amino acids
Theoretical weight: 19.85 KDa
Source organism: Lathyrus ochrus
Expression system: Not provided
UniProt:
  • Canonical: P04122 (Residues: 1-181; Coverage: 100%)
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls
Mannose/glucose-specific lectin alpha 1 chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 52 amino acids
Theoretical weight: 5.78 KDa
Source organism: Lathyrus ochrus
Expression system: Not provided
UniProt:
  • Canonical: P12306 (Residues: 1-52; Coverage: 98%)
Sequence domains: Legume lectin domain

Ligands and Environments

3 bound ligands:
Ligand CA 4 x CA
Ligand MN 4 x MN
Ligand MMA 4 x MMA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 56.3Å b: 139.8Å c: 62.7Å
α: 90° β: 91° γ: 90°
R-values:
R R work R free
0.182 0.182 not available
Expression system: Not provided

Quick links

Citations

8 review citations

Lectin-carbohydrate complexes of plants and animals: an atomic view.
Sharon N. (1993)
7 more

5 mentions without citation

Man-Specific Lectins from Plants, Fungi, Algae and Cyanobacteria, as Potential Blockers for SARS-CoV, MERS-CoV and SARS-CoV-2 (COVID-19) Coronaviruses: Biomedical Perspectives.
Barre et al. (2021)
4 more