› 1lqb

X-ray diffraction
2Å resolution

Crystal structure of a hydroxylated HIF-1 alpha peptide bound to the pVHL/elongin-C/elongin-B complex

Released:
DOI: 10.2210/pdb1lqb/pdb
PDB entry 1lqb coloured by chain, front view.
PDB entry 1lqb coloured by chain, side view.
PDB entry 1lqb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the recognition of hydroxyproline in HIF-1 alpha by pVHL.
Hon WC, Wilson MI, Harlos K, Claridge TD, Schofield CJ, Pugh CW, Maxwell PH, Ratcliffe PJ, Stuart DI, Jones EY
Nature 417 975-8 (2002)
PMID: 12050673

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-154087 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Elongin-B Chain: A
Molecule details ›
Chain: A
Length: 118 amino acids
Theoretical weight: 13.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15370 (Residues: 1-118; Coverage: 100%)
Gene names: ELOB, TCEB2
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Elongin-C Chain: B
Molecule details ›
Chain: B
Length: 96 amino acids
Theoretical weight: 10.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15369 (Residues: 17-112; Coverage: 86%)
Gene names: ELOC, TCEB1
Sequence domains: Skp1 family, tetramerisation domain
Structure domains: Potassium Channel Kv1.1; Chain A
von Hippel-Lindau disease tumor suppressor Chain: C
Molecule details ›
Chain: C
Length: 162 amino acids
Theoretical weight: 18.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40337 (Residues: 54-213; Coverage: 75%)
Gene name: VHL
Sequence domains:
Structure domains:
Hypoxia-inducible factor 1-alpha Chain: D
Molecule details ›
Chain: D
Length: 34 amino acids
Theoretical weight: 3.98 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q16665 (Residues: 549-582; Coverage: 4%)
Gene names: BHLHE78, HIF1A, MOP1, PASD8
Sequence domains: Hypoxia-inducible factor-1

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand HYP 1 x HYP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P43212
Unit cell:
a: 58.94Å b: 58.94Å c: 243.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.277
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

162 review citations

Targeting HIF-1 for cancer therapy.
Semenza GL. (2003)
161 more

23 mentions without citation

Prolyl 4-hydroxylase.
Gorres et al. (2010)
22 more