› 1lr4

X-ray diffraction
2Å resolution

Room Temperature Crystal Structure of the Apo-form of the catalytic subunit of protein kinase CK2 from Zea mays

Released:
DOI: 10.2210/pdb1lr4/pdb
PDB entry 1lr4 coloured by chain, front view.
PDB entry 1lr4 coloured by chain, side view.
PDB entry 1lr4 coloured by chain, top view.
Source organism: Zea mays
Primary publication:
Inclining the purine base binding plane in protein kinase CK2 by exchanging the flanking side-chains generates a preference for ATP as a cosubstrate.
Yde CW, Ermakova I, Issinger OG, Niefind K
J Mol Biol 347 399-414 (2005)
PMID: 15740749

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151243 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Casein kinase II subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 332 amino acids
Theoretical weight: 39.29 KDa
Source organism: Zea mays
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28523 (Residues: 1-332; Coverage: 100%)
Gene name: ACK2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BEN 1 x BEN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: C2
Unit cell:
a: 142.55Å b: 61.44Å c: 45.41Å
α: 90° β: 103.06° γ: 90°
R-values:
R R work R free
0.195 0.193 0.237
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Protein kinases CK1 and CK2 as new targets for neurodegenerative diseases.
Perez et al. (2011)
1 more