› 1ltq

X-ray diffraction
2.33Å resolution

CRYSTAL STRUCTURE OF T4 POLYNUCLEOTIDE KINASE

Released:
DOI: 10.2210/pdb1ltq/pdb
PDB entry 1ltq coloured by chain, front view.
PDB entry 1ltq coloured by chain, side view.
PDB entry 1ltq coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
Structure of a tRNA repair enzyme and molecular biology workhorse: T4 polynucleotide kinase.
Galburt EA, Pelletier J, Wilson G, Stoddard BL
Structure 10 1249-60 (2002)
PMID: 12220496

Function and Biology Details

Reactions catalysed: 
ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA
A deoxyribonucleoside 3'-phosphate + H(2)O = a deoxyribonucleoside + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139258 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polynucleotide kinase Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 35.23 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P06855 (Residues: 1-301; Coverage: 100%)
Gene name: pseT
Sequence domains: AAA domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand DMS 1 x DMS
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: I222
Unit cell:
a: 78.585Å b: 93.684Å c: 124.165Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.236 0.261
Expression system: Escherichia coli

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Citations

7 review citations

Predicting gene function by conserved co-expression.
van Noort et al. (2003)
6 more

5 mentions without citation

Polynucleotide kinase as a potential target for enhancing cytotoxicity by ionizing radiation and topoisomerase I inhibitors.
Bernstein et al. (2008)
4 more