Function and Biology Details
Reaction catalysed:
5,10-methylenetetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- NAD(P)-binding domain superfamily
- Methylene tetrahydromethanopterin dehydrogenase, NADP-binding domain
- Methylene-tetrahydromethanopterin dehydrogenase, N-terminal
- Aminoacid dehydrogenase-like, N-terminal domain superfamily
- Methylene-tetrahydromethanopterin dehydrogenase, N-terminal domain superfamily
Structure analysis Details
Assembly composition:
homo trimer (preferred)
Assembly name:
Bifunctional protein MdtA (preferred)
PDBe Complex ID:
PDB-CPX-157320 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional protein MdtA
Molecule details ›
Chains: A, B, C
Length: 287 amino acids
Theoretical weight: 29.64 KDa
Source organism: Methylorubrum extorquens AM1
Expression system: Escherichia coli
UniProt:
Sequence domains: Methylene-tetrahydromethanopterin dehydrogenase, N-terminal
Structure domains:
Length: 287 amino acids
Theoretical weight: 29.64 KDa
Source organism: Methylorubrum extorquens AM1
Expression system: Escherichia coli
UniProt:
- Canonical:
P55818 (Residues: 2-288; Coverage: 100%)
Sequence domains: Methylene-tetrahydromethanopterin dehydrogenase, N-terminal
Structure domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup:
C2
Expression system: Escherichia coli
