› 1m11

Electron Microscopy
16Å resolution

structural model of human decay-accelerating factor bound to echovirus 7 from cryo-electron microscopy

Released:
DOI: 10.2210/pdb1m11/pdb
PDB entry 1m11 coloured by chain, front view.
PDB entry 1m11 coloured by chain, side view.
PDB entry 1m11 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of decay-accelerating factor bound to echovirus 7: a virus-receptor complex.
He Y, Lin F, Chipman PR, Bator CM, Baker TS, Shoham M, Kuhn RJ, Medof ME, Rossmann MG
Proc Natl Acad Sci U S A 99 10325-9 (2002)
PMID: 12119400

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero 240-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139977 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Complement decay-accelerating factor Chain: R
Molecule details ›
Chain: R
Length: 243 amino acids
Theoretical weight: 27.02 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P08174 (Residues: 35-277; Coverage: 70%)
Gene names: CD55, CR, DAF
Sequence domains: Sushi repeat (SCR repeat)
COAT PROTEIN VP1 Chain: 1
Molecule details ›
Chain: 1
Length: 278 amino acids
Theoretical weight: 31.68 KDa
Source organism: Echovirus E7
Expression system: Homo sapiens
UniProt:
  • Canonical: Q914E0 (Residues: 569-846; Coverage: 13%)
Sequence domains: picornavirus capsid protein
COAT PROTEIN VP2 Chain: 2
Molecule details ›
Chain: 2
Length: 254 amino acids
Theoretical weight: 28.44 KDa
Source organism: Echovirus E7
Expression system: Homo sapiens
UniProt:
  • Canonical: Q914E0 (Residues: 77-330; Coverage: 12%)
Sequence domains: picornavirus capsid protein
COAT PROTEIN VP3 Chain: 3
Molecule details ›
Chain: 3
Length: 238 amino acids
Theoretical weight: 26.26 KDa
Source organism: Echovirus E7
Expression system: Homo sapiens
UniProt:
  • Canonical: Q914E0 (Residues: 331-568; Coverage: 11%)
Sequence domains: picornavirus capsid protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 16Å
Expression systems:
  • Komagataella pastoris
  • Homo sapiens

Quick links

Citations

6 review citations

Coxsackievirus myocarditis: interplay between virus and host in the pathogenesis of heart disease.
Tam PE. (2006)
5 more

1 mention without citation

Structural Basis of Human Parechovirus Neutralization by Human Monoclonal Antibodies.
Shakeel et al. (2015)