› 1m4n

X-ray diffraction
2.01Å resolution

CRYSTAL STRUCTURE OF APPLE ACC SYNTHASE IN COMPLEX WITH [2-(AMINO-OXY)ETHYL](5'-DEOXYADENOSIN-5'-YL)(METHYL)SULFONIUM

Released:
DOI: 10.2210/pdb1m4n/pdb
PDB entry 1m4n coloured by chain, front view.
PDB entry 1m4n coloured by chain, side view.
PDB entry 1m4n coloured by chain, top view.
Source organism: Malus domestica
Primary publication:
Structure of 1-aminocyclopropane-1-carboxylate synthase in complex with an amino-oxy analogue of the substrate: implications for substrate binding.
Capitani G, Eliot AC, Gut H, Khomutov RM, Kirsch JF, Grütter MG
Biochim Biophys Acta 1647 55-60 (2003)
PMID: 12686108

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine = 1-aminocyclopropane-1-carboxylate + S-methyl-5'-thioadenosine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153493 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-aminocyclopropane-1-carboxylate synthase Chain: A
Molecule details ›
Chain: A
Length: 435 amino acids
Theoretical weight: 49.09 KDa
Source organism: Malus domestica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37821 (Residues: 1-435; Coverage: 92%)
Gene name: ACS-1
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
2 bound ligands:
Ligand AAD 1 x AAD
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: C2
Unit cell:
a: 105Å b: 61.33Å c: 76.94Å
α: 90° β: 123.48° γ: 90°
R-values:
R R work R free
0.199 0.199 0.232
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Pyridoxal phosphate enzymes: mechanistic, structural, and evolutionary considerations.
Eliot et al. (2004)
9 other articles

3 mentions without citation

Structural characterization of the Mycobacterium tuberculosis biotin biosynthesis enzymes 7,8-diaminopelargonic acid synthase and dethiobiotin synthetase .
Dey et al. (2010)
2 more