› 1m53

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF ISOMALTULOSE SYNTHASE (PALI) FROM KLEBSIELLA SP. LX3

Released:
DOI: 10.2210/pdb1m53/pdb
PDB entry 1m53 coloured by chain, front view.
PDB entry 1m53 coloured by chain, side view.
PDB entry 1m53 coloured by chain, top view.
Source organism: Klebsiella sp. LX3
Primary publication:
Isomaltulose synthase (PalI) of Klebsiella sp. LX3. Crystal structure and implication of mechanism.
Zhang D, Li N, Lok SM, Zhang LH, Swaminathan K
J Biol Chem 278 35428-34 (2003)
PMID: 12819210

Function and Biology Details

Reaction catalysed: 
Sucrose = 6-O-alpha-D-glucopyranosyl-D-fructofuranose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185205 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 570 amino acids
Theoretical weight: 67.25 KDa
Source organism: Klebsiella sp. LX3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8KR84 (Residues: 29-598; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: P212121
Unit cell:
a: 59.239Å b: 94.153Å c: 111.294Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

α-Amylase: an enzyme specificity found in various families of glycoside hydrolases.
Janeček et al. (2014)
5 more

7 mentions without citation

The 4R's Framework of Nutritional Strategies for Post-Exercise Recovery: A Review with Emphasis on New Generation of Carbohydrates.
Bonilla et al. (2020)
6 more