› 1m5x

X-ray diffraction
2.25Å resolution

Crystal structure of the homing endonuclease I-MsoI bound to its DNA substrate

Released:
DOI: 10.2210/pdb1m5x/pdb
PDB entry 1m5x coloured by chain, front view.
PDB entry 1m5x coloured by chain, side view.
PDB entry 1m5x coloured by chain, top view.
Source organism: Monomastix sp. OKE-1
Primary publication:
Flexible DNA target site recognition by divergent homing endonuclease isoschizomers I-CreI and I-MsoI.
Chevalier B, Turmel M, Lemieux C, Monnat RJ, Stoddard BL
J Mol Biol 329 253-69 (2003)
PMID: 12758074

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110994 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homing endonuclease LAGLIDADG domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 170 amino acids
Theoretical weight: 19.47 KDa
Source organism: Monomastix sp. OKE-1
Expression system: Escherichia coli
UniProt:
  • Canonical: C0JWR6 (Residues: 1-170; Coverage: 100%)
Gene name: orf170
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
5'-D(*GP*CP*AP*GP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*CP*CP*G)-3' Chain: C
Molecule details ›
Chain: C
Length: 24 nucleotides
Theoretical weight: 7.42 KDa
Source organism: Monomastix sp. OKE-1
Expression system: Not provided
5'-D(*CP*GP*GP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*TP*CP*TP*GP*C)-3' Chain: D
Molecule details ›
Chain: D
Length: 24 nucleotides
Theoretical weight: 7.32 KDa
Source organism: Monomastix sp. OKE-1
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P1
Unit cell:
a: 41.51Å b: 42.21Å c: 71.32Å
α: 73.34° β: 73.17° γ: 71.09°
R-values:
R R work R free
0.219 0.219 0.255
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy.
Silva et al. (2011)
8 more

4 mentions without citation

Computational reprogramming of homing endonuclease specificity at multiple adjacent base pairs.
Ashworth et al. (2010)
3 more