› 1m61

X-ray diffraction
2.5Å resolution

Crystal structure of the apo SH2 domains of ZAP-70

Released:
DOI: 10.2210/pdb1m61/pdb
PDB entry 1m61 coloured by chain, front view.
PDB entry 1m61 coloured by chain, side view.
PDB entry 1m61 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure and NMR studies of the apo SH2 domains of ZAP-70: two bikes rather than a tandem.
Folmer RH, Geschwindner S, Xue Y
Biochemistry 41 14176-84 (2002)
PMID: 12450381

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155055 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase ZAP-70 Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 29.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P43403 (Residues: 1-256; Coverage: 41%)
Gene names: SRK, ZAP70
Sequence domains: SH2 domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P21
Unit cell:
a: 48.851Å b: 52.227Å c: 54.455Å
α: 90° β: 89.91° γ: 90°
R-values:
R R work R free
0.245 0.245 0.286
Expression system: Escherichia coli BL21(DE3)

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Citations

9 review citations

Tec family kinases in T lymphocyte development and function.
Berg et al. (2005)
8 more

7 mentions without citation

DockoMatic 2.0: high throughput inverse virtual screening and homology modeling.
Bullock et al. (2013)
6 more