› 1m6d

X-ray diffraction
1.7Å resolution

Crystal structure of human cathepsin F

Released:
DOI: 10.2210/pdb1m6d/pdb
PDB entry 1m6d coloured by chain, front view.
PDB entry 1m6d coloured by chain, side view.
PDB entry 1m6d coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of human cathepsin F and its implications for the development of novel immunomodulators.
Somoza JR, Palmer JT, Ho JD
J Mol Biol 322 559-68 (2002)
PMID: 12225749

Function and Biology Details

Reaction catalysed: 
The recombinant enzyme cleaves synthetic substrates with Phe and Leu (better than Val) in P2, with high specificity constant (k(cat)/K(m)) comparable to that of cathepsin L.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193688 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cathepsin F Chains: A, B
Molecule details ›
Chains: A, B
Length: 214 amino acids
Theoretical weight: 23.66 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9UBX1 (Residues: 271-484; Coverage: 46%)
Gene name: CTSF
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases

Ligands and Environments

1 bound ligand:
Ligand MYP 2 x MYP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21212
Unit cell:
a: 68.25Å b: 103.26Å c: 58.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.195 0.222
Expression system: Komagataella pastoris

Quick links

Citations

4 review citations

Metal and redox modulation of cysteine protein function.
Giles et al. (2003)
3 more

14 mentions without citation

Cysteine cathepsins: from structure, function and regulation to new frontiers.
Turk et al. (2012)
13 more