› 1m7s

X-ray diffraction
1.8Å resolution

Crystal Structure Analysis of Catalase CatF of Pseudomonas syringae

Released:
DOI: 10.2210/pdb1m7s/pdb
PDB entry 1m7s coloured by chain, front view.
PDB entry 1m7s coloured by chain, side view.
PDB entry 1m7s coloured by chain, top view.
Source organism: Pseudomonas syringae
Primary publication:
Structure of the Clade 1 catalase, CatF of Pseudomonas syringae, at 1.8 A resolution.
Carpena X, Soriano M, Klotz MG, Duckworth HW, Donald LJ, Melik-Adamyan W, Fita I, Loewen PC
Proteins 50 423-36 (2003)
PMID: 12557185

Function and Biology Details

Reaction catalysed: 
2 H(2)O(2) = O(2) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155449 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 484 amino acids
Theoretical weight: 54.04 KDa
Source organism: Pseudomonas syringae
Expression system: Escherichia coli
UniProt:
  • Canonical: P46206 (Residues: 27-510; Coverage: 100%)
Gene names: catF, katB
Sequence domains:
Structure domains: Catalase core domain

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 60.63Å b: 153.87Å c: 109.19Å
α: 90° β: 102.8° γ: 90°
R-values:
R R work R free
0.183 0.187 0.24
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Human catalase: looking for complete identity.
Goyal et al. (2010)
2 more

4 mentions without citation

Monofunctional Heme-Catalases.
Hansberg W. (2022)
3 more