› 1m8w

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF THE PUMILIO-HOMOLOGY DOMAIN FROM HUMAN PUMILIO1 IN COMPLEX WITH NRE1-19 RNA

Released:
DOI: 10.2210/pdb1m8w/pdb
PDB entry 1m8w coloured by chain, front view.
PDB entry 1m8w coloured by chain, side view.
PDB entry 1m8w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Modular recognition of RNA by a human pumilio-homology domain.
Wang X, McLachlan J, Zamore PD, Hall TM
Cell 110 501-12 (2002)
PMID: 12202039

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-219596 (preferred)
Entry contents:
1 distinct polypeptide molecule
4 distinct RNA molecules
Macromolecules (5 distinct):
Pumilio homolog 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 349 amino acids
Theoretical weight: 40.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14671 (Residues: 828-1176; Coverage: 29%)
Gene names: KIAA0099, PUM1, PUMH1
Sequence domains: Pumilio-family RNA binding repeat
Structure domains: Leucine-rich Repeat Variant
5'-R(P*UP*UP*GP*UP*AP*UP*AP*U)-3' Chain: C
Molecule details ›
Chain: C
Length: 8 nucleotides
Theoretical weight: 2.49 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-R(P*UP*GP*UP*AP*UP*AP*U)-3' Chain: D
Molecule details ›
Chain: D
Length: 7 nucleotides
Theoretical weight: 2.18 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-R(P*UP*UP*GP*UP*CP*CP*AP*G)-3' Chain: E
Molecule details ›
Chain: E
Length: 8 nucleotides
Theoretical weight: 2.5 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-R(P*UP*GP*UP*CP*CP*AP*G)-3' Chain: F
Molecule details ›
Chain: F
Length: 7 nucleotides
Theoretical weight: 2.2 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: C2
Unit cell:
a: 262.73Å b: 37.73Å c: 82.52Å
α: 90° β: 102.95° γ: 90°
R-values:
R R work R free
0.228 0.224 0.289
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

75 review citations

Gene regulation by long non-coding RNAs and its biological functions.
Statello et al. (2021)
74 more

16 mentions without citation

How RNA-Binding Proteins Interact with RNA: Molecules and Mechanisms.
Corley et al. (2020)
15 more