› 1m93

X-ray diffraction
1.65Å resolution

1.65 A Structure of Cleaved Viral Serpin CRMA

Released:
DOI: 10.2210/pdb1m93/pdb
PDB entry 1m93 coloured by chain, front view.
PDB entry 1m93 coloured by chain, side view.
PDB entry 1m93 coloured by chain, top view.
Source organism: Cowpox virus
Primary publication:
Crystal structure of viral serpin crmA provides insights into its mechanism of cysteine proteinase inhibition.
Simonovic M, Volz K
Protein Sci 9 1423-7 (2000)
PMID: 10975564

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139578 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Serine proteinase inhibitor 2 Chain: A
Molecule details ›
Chain: A
Length: 55 amino acids
Theoretical weight: 6.08 KDa
Source organism: Cowpox virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07385 (Residues: 1-55; Coverage: 16%)
Gene names: CPXV207, OPG199, SPI-2
Structure domains: Helix hairpin bin
Serine proteinase inhibitor 2 Chain: B
Molecule details ›
Chain: B
Length: 245 amino acids
Theoretical weight: 27.4 KDa
Source organism: Cowpox virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07385 (Residues: 56-300; Coverage: 72%)
Gene names: CPXV207, OPG199, SPI-2
Sequence domains: Serpin (serine protease inhibitor)
Structure domains:
Serine proteinase inhibitor 2 Chain: C
Molecule details ›
Chain: C
Length: 41 amino acids
Theoretical weight: 4.52 KDa
Source organism: Cowpox virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07385 (Residues: 301-341; Coverage: 12%)
Gene names: CPXV207, OPG199, SPI-2

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 49.52Å b: 92.59Å c: 100.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 0.245
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The protein structures that shape caspase activity, specificity, activation and inhibition.
Fuentes-Prior et al. (2004)
7 more

2 mentions without citation

Poxvirus proteomics and virus-host protein interactions.
Van Vliet et al. (2009)
1 more