› 1m9y

X-ray diffraction
1.9Å resolution

X-ray crystal structure of Cyclophilin A/HIV-1 CA N-terminal domain (1-146) M-type H87A,G89A Complex.

Released:
DOI: 10.2210/pdb1m9y/pdb
PDB entry 1m9y coloured by chain, front view.
PDB entry 1m9y coloured by chain, side view.
PDB entry 1m9y coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into the catalytic mechanism of cyclophilin A.
Howard BR, Vajdos FF, Li S, Sundquist WI, Hill CP
Nat Struct Biol 10 475-81 (2003)
PMID: 12730686

Function and Biology Details

Reaction catalysed: 
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158799 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peptidyl-prolyl cis-trans isomerase A Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 165 amino acids
Theoretical weight: 18.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62937 (Residues: 1-165; Coverage: 100%)
Gene names: CYPA, PPIA
Sequence domains: Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Structure domains: Cyclophilin-like
HIV-1 Capsid Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 146 amino acids
Theoretical weight: 16.15 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72497 (Residues: 133-278; Coverage: 29%)
Gene name: gag
Sequence domains: gag protein p24 N-terminal domain
Structure domains: Human Immunodeficiency Virus Type 1 Capsid Protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P1
Unit cell:
a: 38.426Å b: 111.223Å c: 67.78Å
α: 89.99° β: 101.45° γ: 89.75°
R-values:
R R work R free
0.169 0.162 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Prolyl cis-trans isomerization as a molecular timer.
Lu et al. (2007)
11 more

1 mention without citation

Catalytic mechanism of cyclophilin as observed in molecular dynamics simulations: pathway prediction and reconciliation of X-ray crystallographic and NMR solution data.
Trzesniak et al. (2006)