› 1mb9

X-ray diffraction
2.11Å resolution

BETA-LACTAM SYNTHETASE COMPLEXED WITH ATP

Released:
DOI: 10.2210/pdb1mb9/pdb
PDB entry 1mb9 coloured by chain, front view.
PDB entry 1mb9 coloured by chain, side view.
PDB entry 1mb9 coloured by chain, top view.
Source organism: Streptomyces clavuligerus
Primary publication:
The catalytic cycle of beta -lactam synthetase observed by x-ray crystallographic snapshots.
Miller MT, Bachmann BO, Townsend CA, Rosenzweig AC
Proc Natl Acad Sci U S A 99 14752-7 (2002)
PMID: 12409610

Function and Biology Details

Reaction catalysed: 
ATP + L-N(2)-(2-carboxyethyl)arginine = AMP + diphosphate + deoxyamidinoproclavaminate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143602 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxyethyl-arginine beta-lactam-synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 513 amino acids
Theoretical weight: 54.6 KDa
Source organism: Streptomyces clavuligerus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DJQ7 (Residues: 1-513; Coverage: 100%)
Gene name: bls
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 4 x MG
Ligand POP 1 x POP
Ligand AMP 1 x AMP
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 61.252Å b: 97.571Å c: 81.313Å
α: 90° β: 90.69° γ: 90°
R-values:
R R work R free
0.21 0.21 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Asparagine synthetase chemotherapy.
Richards et al. (2006)
9 more

2 mentions without citation

Distinct Conformation of ATP Molecule in Solution and on Protein.
Kobayashi et al. (2013)
1 more