› 1mg5

X-ray diffraction
1.63Å resolution

Crystal structure of Drosophila melanogaster alcohol dehydrogenase complexed with NADH and acetate at 1.6 A

Released:
DOI: 10.2210/pdb1mg5/pdb
PDB entry 1mg5 coloured by chain, front view.
PDB entry 1mg5 coloured by chain, side view.
PDB entry 1mg5 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Drosophila alcohol dehydrogenase: acetate-enzyme interactions and novel insights into the effects of electrostatics on catalysis.
Benach J, Winberg JO, Svendsen JS, Atrian S, Gonzàlez-Duarte R, Ladenstein R
J Mol Biol 345 579-98 (2005)
PMID: 15581900

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132408 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 27.66 KDa
Source organism: Drosophila melanogaster
UniProt:
  • Canonical: P00334 (Residues: 2-256; Coverage: 100%)
Gene names: Adh, CG3481
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAI 2 x NAI
1 bound ligand:
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P21
Unit cell:
a: 47.481Å b: 69.519Å c: 75.923Å
α: 90° β: 89.13° γ: 90°
R-values:
R R work R free
0.168 0.168 0.205

Quick links

Citations

1 review citation

Systems genetics of alcoholism.
Sloan et al. (2008)
14 other articles

4 mentions without citation

Evolving protein functional diversity in new genes of Drosophila.
Zhang et al. (2004)
3 more