› 1mgv

X-ray diffraction
2.1Å resolution

Crystal Structure of the R391A Mutant of 7,8-Diaminopelargonic Acid Synthase

Released:
DOI: 10.2210/pdb1mgv/pdb
PDB entry 1mgv coloured by chain, front view.
PDB entry 1mgv coloured by chain, side view.
PDB entry 1mgv coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The dual-specific active site of 7,8-diaminopelargonic acid synthase and the effect of the R391A mutation.
Eliot AC, Sandmark J, Schneider G, Kirsch JF
Biochemistry 41 12582-9 (2002)
PMID: 12379100

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146421 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 429 amino acids
Theoretical weight: 47.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12995 (Residues: 1-429; Coverage: 100%)
Gene names: JW0757, b0774, bioA
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
2 bound ligands:
Ligand NA 2 x NA
Ligand IPA 4 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: C2
Unit cell:
a: 127.88Å b: 55.899Å c: 116.237Å
α: 90° β: 110.06° γ: 90°
R-values:
R R work R free
0.203 0.201 0.233
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Bioinformatic analysis of a PLP-dependent enzyme superfamily suitable for biocatalytic applications.
Steffen-Munsberg et al. (2015)
1 more

1 mention without citation

Analysis of Polyphenol Extract from Hazel Leaf and Ameliorative Efficacy and Mechanism against Hyperuricemia Zebrafish Model via Network Pharmacology and Molecular Docking.
Wang et al. (2024)