› 1mi5

X-ray diffraction
2.5Å resolution

The crystal structure of LC13 TcR in complex with HLAB8-EBV peptide complex

Released:
DOI: 10.2210/pdb1mi5/pdb
PDB entry 1mi5 coloured by chain, front view.
PDB entry 1mi5 coloured by chain, side view.
PDB entry 1mi5 coloured by chain, top view.
Source organisms:
Primary publication:
A structural basis for the selection of dominant alphabeta T cell receptors in antiviral immunity.
Kjer-Nielsen L, Clements CS, Purcell AW, Brooks AG, Whisstock JC, Burrows SR, McCluskey J, Rossjohn J
Immunity 18 53-64 (2003)
PMID: 12530975

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-134722 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, B alpha chain Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 32.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01889 (Residues: 25-301; Coverage: 82%)
Gene names: HLA-B, HLAB
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Epstein-Barr nuclear antigen 3 Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.05 KDa
Source organism: human gammaherpesvirus 4
Expression system: Not provided
UniProt:
  • Canonical: Q3KST2 (Residues: 325-333; Coverage: 1%)
Gene names: BLRF3-BERF1, EBNA3
T cell receptor alpha chain constant Chain: D
Molecule details ›
Chain: D
Length: 201 amino acids
Theoretical weight: 22.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01848 (Residues: 1-89; Coverage: 64%)
Gene names: TCRA, TRAC
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins
T cell receptor beta constant 1 Chain: E
Molecule details ›
Chain: E
Length: 241 amino acids
Theoretical weight: 27.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01850 (Residues: 1-129; Coverage: 73%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 56.49Å b: 105.9Å c: 144.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.288
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

58 review citations

How TCRs bind MHCs, peptides, and coreceptors.
Rudolph et al. (2006)
57 more

45 mentions without citation

The molecular basis of TCR germline bias for MHC is surprisingly simple.
Garcia et al. (2009)
44 more