› 1mj1

Electron Microscopy
13Å resolution

FITTING THE TERNARY COMPLEX OF EF-Tu/tRNA/GTP AND RIBOSOMAL PROTEINS INTO A 13 A CRYO-EM MAP OF THE COLI 70S RIBOSOME

Released:
DOI: 10.2210/pdb1mj1/pdb
PDB entry 1mj1 coloured by chain, front view.
PDB entry 1mj1 coloured by chain, side view.
PDB entry 1mj1 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Ribosome interactions of aminoacyl-tRNA and elongation factor Tu in the codon-recognition complex.
Stark H, Rodnina MV, Wieden HJ, Zemlin F, Wintermeyer W, van Heel M
Nat Struct Biol 9 849-54 (2002)
PMID: 12379845
Related structures: EMD-1004

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-151451 (preferred)
Entry contents:
4 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (7 distinct):
Elongation factor Tu Chain: A
Molecule details ›
Chain: A
Length: 405 amino acids
Theoretical weight: 46.06 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: Q01698 (Residues: 2-406; Coverage: 100%)
Gene names: tuf, tufA
Sequence domains:
Small ribosomal subunit protein uS12 Chain: O
Molecule details ›
Chain: O
Length: 135 amino acids
Theoretical weight: 14.92 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: Q5SHN3 (Residues: 1-132; Coverage: 100%)
Gene names: TTHA1697, rpsL
Sequence domains: Ribosomal protein S12/S23
Small ribosomal subunit protein uS13 Chain: P
Molecule details ›
Chain: P
Length: 126 amino acids
Theoretical weight: 14.34 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P80377 (Residues: 1-126; Coverage: 100%)
Gene names: TTHA1667, rps13, rpsM
Sequence domains: Ribosomal protein S13/S18
Large ribosomal subunit protein uL11 Chain: L
Molecule details ›
Chain: L
Length: 141 amino acids
Theoretical weight: 15.11 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P29395 (Residues: 1-141; Coverage: 100%)
Gene names: TM_0454, rplK
Sequence domains:
Phe-tRNA Chains: C, D
Molecule details ›
Chains: C, D
Length: 76 nucleotides
Theoretical weight: 24.89 KDa
Sequence domains: tRNA
sarcin-ricin loop of 23SrRNA Chain: Q
Molecule details ›
Chain: Q
Length: 41 nucleotides
Theoretical weight: 13.37 KDa
helix 69 of 23S rRNA Chain: R
Molecule details ›
Chain: R
Length: 27 nucleotides
Theoretical weight: 8.69 KDa

Ligands and Environments

No bound ligands
11 modified residues:
Ligand 2MG 2 x 2MG
Ligand H2U 4 x H2U
Ligand M2G 2 x M2G
Ligand OMC 2 x OMC
Ligand OMG 2 x OMG
Ligand YG 2 x YG
Ligand PSU 4 x PSU
Ligand 5MC 4 x 5MC
Ligand 7MG 2 x 7MG
Ligand 5MU 2 x 5MU
Ligand 1MA 2 x 1MA

Experiments and Validation Details

Entry percentile scores
Resolution: 13Å
Relevant EMDB volumes: EMD-1004

Quick links

Citations

27 review citations

Structural insights into translational fidelity.
Ogle et al. (2005)
26 more

3 mentions without citation

The ribosome structure controls and directs mRNA entry, translocation and exit dynamics.
Kurkcuoglu et al. (2008)
2 more