› 1mla

X-ray diffraction
1.5Å resolution

THE ESCHERICHIA COLI MALONYL-COA:ACYL CARRIER PROTEIN TRANSACYLASE AT 1.5-ANGSTROMS RESOLUTION. CRYSTAL STRUCTURE OF A FATTY ACID SYNTHASE COMPONENT

Released:
DOI: 10.2210/pdb1mla/pdb
PDB entry 1mla coloured by chain, front view.
PDB entry 1mla coloured by chain, side view.
PDB entry 1mla coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The Escherichia coli malonyl-CoA:acyl carrier protein transacylase at 1.5-A resolution. Crystal structure of a fatty acid synthase component.
Serre L, Verbree EC, Dauter Z, Stuitje AR, Derewenda ZS
J Biol Chem 270 12961-4 (1995)
PMID: 7768883

Function and Biology Details

Reaction catalysed: 
Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141990 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malonyl CoA-acyl carrier protein transacylase Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 32.44 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AAI9 (Residues: 1-309; Coverage: 100%)
Gene names: JW1078, b1092, fabD, tfpA
Sequence domains: Acyl transferase domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P65
Unit cell:
a: 68.18Å b: 68.18Å c: 118.56Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.184 not available
Expression system: Escherichia coli

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Citations

22 review citations

The structural biology of type II fatty acid biosynthesis.
White et al. (2005)
21 more

49 mentions without citation

Engineering the acyltransferase substrate specificity of assembly line polyketide synthases.
Dunn et al. (2013)
48 more